ToCLEM: Merging Fluorescent RNA FISH and Protein Labeling with Sub-cellular Ultrastructure by Electron Microscopy

Abigail Lytton-Jean is the Scientific Director of the Nanotechnology for Integrative BioEM (NanoFIB) shared resource at the Koch Institute for Integrative Cancer Research at MIT. Her team serves as an innovation platform focused on the characterization and imaging of biologically relevant materials, with unique expertise in multi-scale electron microscopy (EM) under both room-temperature and cryogenic conditions. Central to NanoFIB’s impact are bespoke, custom-tailored approaches to workflow design, protocol development, and sample preparation that enable advanced volume EM, cryoEM, correlative EM, and immunoEM applications. These integrated preparation and imaging workflows support multiscale structural visualization of cells, tissues, and synthetic materials while enabling precise targeting of regions of interest with nanometer-scale resolution.

Dr. Lytton-Jean received her Ph.D. in Chemistry from Northwestern University under the supervision of Prof. Chad Mirkin. She completed her postdoctoral training at MIT in the laboratories of Prof. Robert Langer and Prof. Daniel Anderson, where she specialized in bio-nanotechnology and drug delivery